Biochem/physiol Actions

ED₅₀ = 5-15 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

General description

Recombinant, human amphiregulin (AR) consisting of amino acids 101-198 and expressed in E. coli. Human amphiregulin is able to stimulate the proliferation of various human and mouse keratinocytes, mammary epithelial cells, and some fibroblasts. Shown to be an autocrine growth factor, amphiregulin is also a growth inhibitor for various tumor cells and can bind to the EGF receptor.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Warning

Toxicity: Standard Handling (A)